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Domain experts in cytometry laboratories and core facilities increasingly recognize the need for automated workflows in the face of increasing data complexity, but by and large, still conduct all analysis using traditional applications, predominantly FlowJo.

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7Duke Human Vaccine Institute, Durham, NC, United StatesĪn important challenge for primary or secondary analysis of cytometry data is how to facilitate productive collaboration between domain and quantitative experts.

6Department of Surgery, Duke University Medical Center, Durham, NC, United States.

5Duke Immune Profiling Core, Duke University School of Medicine, Durham, NC, United States.

4BD Life Sciences - FlowJo, Ashland, OR, United States.

3Center for Human Systems Immunology, Duke University Medical Center, Durham, NC, United States.

2Department of Biostatistics and Bioinformatics, Duke University Medical Center, Durham, NC, United States.

1Duke Center for AIDS Research, Duke University, Durham, NC, United States.

Weinhold 1,5,6, Guido Ferrari 1,3,6 and Cliburn Chan 1,2,3 Sample Get Info has a new dialog window interface, with new capabilities (like showing a table of keywords in all samples in the group showing only interesting keywords showing only keywords with distinct values.)įlowJo will notify the user if a Table or Layout Batch operation points to a group that has been deleted.įlowJo now shows 9 decade log scale for Yeti files.įixed reading certain XML workspace files.įixed crash while editing compensation matrix (from v9.9.Scott White 1,2,3*, John Quinn 4, Jennifer Enzor 5,6, Janet Staats 5,6, Sarah M. (Change this behavior by global preference, or at a single time by holding down the option key when clicking on the menu.)Ĭompensation Wizard displays the SSM with a heat map background to highlight parameter pairs with high spillover-spreading. Parameters selection for tSNE, LDA, PCA, and layout editor N x N plots made more powerful.īy default, most menus will no longer show uncompensated fluorescence parameters for compensated files. Layout Editor accepts text pastes (and will create text object or table if tabular format) LDA (linear discriminant analysis - optimal separation of multiple subsets) Native support for G3,4,5 and all Intel based systems. *FlowJo v9 is NOT currently compatible with macOS v. We are working on a new version of FlowJo™ that will use the current version of Java.

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This update removes Java 6, which is required by FlowJo versions 10.2 and older.

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This new version of the Mac OS supports Java 7 and higher.

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Sierra, macOS 10.12, is now available as a free upgrade. (Downsample before you concatenate if desired.)

Removed the option to downsample when concatenating files.

Unchecked 'Display other X/Y parameters than in parent plot' as default in Gates Preferences.

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Updated EULA to raise the awareness of the fact that you may not install FlowJo on a VM.

Better interface for Licensing Preferences Panel in the case where a computer has many hardware addresses.

Added a field to allow specification of R's path in prefs (Diagnostic Panel).

Increased the number of parameters that FlowJo can analyze to 1000.

You'll find these as 'plugins' in the populations band - each offers one-click access to powerful tools to reduce any number of parameters to two and to query a database to name unknown populations. FlowJo 10.2 includes a set of tools for discovery including downsampling, tSNE and CellOntology. The open plugin architecture of 10.2 allows access to cutting edge tools that can be integrated into your current analysis workflows, and we will be releasing developer documentation so any tool can be integrated into FlowJo. Be sure your version of FlowJo Enterprise is new enough before installing this version of FlowJo.įlowJo can now use plugins that can perform a variety of functions, including the creation of populations, creation of derived parameters, and actions when opening or saving a workspace. Note: This version of FlowJo is compatible with FlowJo Enterprise v2.0 or the newly released FlowJo Enterprise 2.1.